分泌型卷曲相關(guān)蛋白5(SFRP5)重組蛋白
Recombinant Secreted Frizzled Related Protein 5 (SFRP5)
SARP3; Secreted Apoptosis Related Protein 3; Frizzled-related protein 1b
- 編號URPC842Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量14.8kDa
- 實際分子量15kDa(差異分析請參閱說明書)
- 片段與標簽Ser45~Val162 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點6.6
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應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 504 ¥ 1260 ¥ 2520 ¥ 7560 ¥ 18900
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPC842Ra01 | 分泌型卷曲相關(guān)蛋白5(SFRP5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC842Ra01 | 分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體 | WB |
| ULAC842Ra71 | 分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAC842Ra21 | 分泌型卷曲相關(guān)蛋白5(SFRP5)單克隆抗體 | WB; IHC; ICC; IP. |
| USEC842Ra | 分泌型卷曲相關(guān)蛋白5(SFRP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC842Ra | 分泌型卷曲相關(guān)蛋白5(SFRP5)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Diabetes Research and Clinical Practice | Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance [PubMed: 23290274] |
| European Journal of Clinical Investigation | Sfrp5 correlates with insulin resistance and oxidative stress [PubMed: 23398169] |
| Pediatric Research | Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children [Pubmed: 24603290] |
| Clin Endocrinol (Oxf). | SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children [Pubmed: 24330025] |
| The Journal of Clinical Endocrinology & Metabolism | Circulating Sfrp5 is a signature of obesity-related metabolic disorders and is regulated by glucose and liraglutide in humans [Pubmed: 23185036] |
| Atherosclerosis | Low serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery disease [Pubmed: 24530778] |
| PLoS One. | Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation [Pubmed:Pmc4072672] |
| J Clin Endocrinol Metab. | Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation [Pubmed:24840810] |
| Oncology Letters | Serum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinoma [Pubmed:25120720] |
| Diabetes Metab Res Rev. | Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls [Pubmed:25139699] |
| Clinical & Experimental Immunology | The wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsis [Pubmed:25382802] |
| Diabetes Metab Res Rev | Circulating SFRP5 levels are elevated in drug-na [PubMed: 25139699] |
| J Matern Fetal Neonatal Med | Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels [PubMed: 26100762] |
| Eur J Clin Invest | Sfrp5 associates with beta-cell function in humans [Pubmed:27019073] |
| Osteoporosis International | Bone marrow sFRP5 level is negatively associated with bone formation markers [pubmed:27986984] |
| Diabetes/Metabolism Research and Reviews | Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults [pubmed:28303637] |
| Cardiovascular Diabetology | Inverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 study [pubmed:28851362] |
| Arteriosclerosis, Thrombosis, and?Vascular Biology? | Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With?… [Pubmed:29674475] |
| Obesity | Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans [Pubmed:29427376] |
| J Bas Res Med Sci | Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats [article-1-352-en.pdf] |
| Molecular?Biology?Reports | The effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndrome [Pubmed: 30194556] |
| Macrophage LRP1 promotes diet-induced hepatic inflammation and metabolic dysfunction by modulating Wnt signaling [] | |
| British?Journal Of Nutrition | A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile [Pubmed: 30375290] |
| J Clin Endocrinol Metab | Role of wnt5a in Metabolic Inflammation in Humans [Pubmed: 30137542] |
| Frontiers in Endocrinology | Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients [Pubmed: 32300333] |
| CLINICAL BIOCHEMISTRY | Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease [Pubmed: 33188773] |
| Sao Paulo Medical Journal | Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … [Pubmed: 32491086] |
| Kidney Blood Press Res | Secreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway [34469882] |
| Cytokine | Association of serum uromodulin with adipokines in dependence of type 2 diabetes [34920231] |
| PLoS One | Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study [Pubmed:34990470] |
| JMIR Res Protoc | Investigating New Sensory Methods Related to Taste Sensitivity, Preferences, and Diet of Mother-Infant Pairs and Their Relationship With Body Composition?… [Pubmed:35475790] |
| Diabetology | Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop?… [] |
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