70kDa熱休克蛋白5(HSPA5)多克隆抗體
Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)
MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78
- 編號UPAC343Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPC343Ra01-70kDa熱休克蛋白5(HSPA5)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.26mg/ml
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 342 ¥ 798 ¥ 1140 ¥ 2850 ¥ 11400
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat HSPA5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebellum Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat HSPA5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Testis Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat HSPA5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: Mouse Testis lysate; Lane3: A549 cell lysate; Primary Ab: 0.1μg/ml Rabbit Anti-Rat HSPA5 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對HSPA5的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別HSPA5。
用法
Western blotting: 0.5-2μg/mL;1:130-520
Immunohistochemistry: 5-20μg/mL;1:13-52
Immunocytochemistry: 5-20μg/mL;1:13-52
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPC343Ra01 | 70kDa熱休克蛋白5(HSPA5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC343Ra01 | 70kDa熱休克蛋白5(HSPA5)多克隆抗體 | WB; IHC |
| UMAC343Ra21 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB; IHC; ICC; IP. |
| USEC343Ra | 70kDa熱休克蛋白5(HSPA5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC343Ra | 70kDa熱休克蛋白5(HSPA5)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSC343Ra01 | 70kDa熱休克蛋白5(HSPA5)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| 41 | Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. [PubMed: 20522592] |
| pharmacological research | Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors [Pubmed: 31048034] |
| EPILEPSY & BEHAVIOR | Fenfluramine acts as a positive modulator of sigma-1 receptors [Pubmed: 32169824] |
| Journal of Oncology | Using Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer [33381181] |
| Heliyon | Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior [] |
| Medicine (Baltimore) | Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients [34414940] |
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