組蛋白脫乙?；?(HDAC1)單克隆抗體
Monoclonal Antibody to Histone Deacetylase 1 (HDAC1)
HDAC1; GON10; HD1; RPD3; RPD3L1
- 編號(hào)UMAC206Hu23
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))E31
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPC206Hu01-組蛋白脫乙?；?(HDAC1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應(yīng)用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 344 ¥ 802 ¥ 1146 ¥ 2865 ¥ 11460
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Stomach Tissue; Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Rectum Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Stomach cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 10μg/ml Mouse Anti-Human HDAC1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: Hela cell
Primary Ab: 30μg/ml Mouse Anti-Human HDAC1 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: A549 cell lysate; Lane3: HepG2 cell lysate Primary Ab: 0.2μg/ml Mouse Anti-Human HDAC1 Antibody Second Ab: 0.2μg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Human Jurkat cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human HDAC1 Monoclonal Antibody (Catalog MAC206Hu23, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)HDAC1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別HDAC1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-30μg/mL;
Flow cytometry: 10μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPC206Hu01 | 組蛋白脫乙?；?(HDAC1)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPC206Hu01 | 組蛋白脫乙?；?(HDAC1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC206Hu01 | 組蛋白脫乙?；?(HDAC1)多克隆抗體 | WB; ICC/IF |
| UMAC206Hu23 | 組蛋白脫乙酰基酶1(HDAC1)單克隆抗體 | WB; IHC; ICC/IF; FCM |
| UMAC206Hu24 | 組蛋白脫乙?；?(HDAC1)單克隆抗體 | WB; IHC; FCM |
| UMAC206Hu25 | 組蛋白脫乙?；?(HDAC1)單克隆抗體 | WB; IHC |
| UMAC206Hu26 | 組蛋白脫乙?；?(HDAC1)單克隆抗體 | WB; IHC |
| UMAC206Hu27 | 組蛋白脫乙酰基酶1(HDAC1)單克隆抗體 | WB; IHC; ICC/IF |
| UMAC206Hu28 | 組蛋白脫乙?；?(HDAC1)單克隆抗體 | WB |
| UMAC206Hu29 | 組蛋白脫乙?；?(HDAC1)單克隆抗體 | IHC |
| UMAC206Hu22 | 組蛋白脫乙酰基酶1(HDAC1)單克隆抗體 | WB; IHC; ICC/IF; FCM |
| UMAC206Hu21 | 組蛋白脫乙?；?(HDAC1)單克隆抗體 | WB; IHC; ICC/IF |
| UFAC206Hu02 | 抗組蛋白脫乙?；?(HDAC1)單克隆抗體 | FCM |
| UFAC206Hu08 | 抗組蛋白脫乙?；?(HDAC1)單克隆抗體 | FCM |
| UFAC206Hu06 | 抗組蛋白脫乙?；?(HDAC1)單克隆抗體 | FCM |
| UFAC206Hu04 | 抗組蛋白脫乙酰基酶1(HDAC1)單克隆抗體 | FCM |
| USEC206Hu | 組蛋白脫乙?；?(HDAC1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC206Hu | 組蛋白脫乙酰基酶1(HDAC1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPGC206Hu01 | 組蛋白脫乙?；?(HDAC1)引物對(duì) | PCR |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Integrative Medicine | Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase [Pubmed:25609369] |
| Tzu Chi Medical Journal | Effects of tetrandrine and caffeine on cell viability and expression of mammalian target of rapamycin, phosphatase and tensin homolog, histone deacetylase 1, and histone acetyltransferase in glioma cells [Science: Article] |
| Restor Neurol Neurosci | Uridine protects against hypoxic-ischemic brain injury by reducing histone deacetylase activity in neonatal rats [PubMed: 26410212] |
| Pharmacol Biochem Behav | Lacosamide reduces HDAC levels in the brain and improves memory: Potential for treatment of Alzheimer's disease [PubMed: 25931268] |
| 網(wǎng)頁打不開 | Effects of caffeine on cell viability and activity of histone deacetylase 1 and histone acetyltransferase in glioma cells [S1016319016300295] |
