70kDa熱休克蛋白5(HSPA5)單克隆抗體
Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)
MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78
- 編號UMAC343Mu21
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C5
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPC343Mu01-70kDa熱休克蛋白5(HSPA5)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 347 ¥ 809 ¥ 1155 ¥ 2888 ¥ 11550
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Mouse Anti-Mouse HSPA5 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Samples: Mouse Cerebrum Tissue;
Primary Ab: 20μg/ml Mouse Anti-Mouse HSPA5 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Samples: Mouse Testis Tissue;
Primary Ab: 20μg/ml Mouse Anti-Mouse HSPA5 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Mouse HSPA5 Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
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Western Blot; Sample: Lane1: Mouse Spinal cord lysate; Lane2: Mouse Liver lysate; Lane3: HepG2 cell lysate Primary Ab: 2μg/ml Mouse Anti-Mouse HSPA5 Antibody Second Ab: 0.2μg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Cerebrum lysate; Lane3: Rat Testis lysate; Lane4: A549 cell lysate
Primary Ab: 0.2μg/ml Mouse Anti-Mouse HSPA5 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對HSPA5的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別HSPA5。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPC343Mu02 | 70kDa熱休克蛋白5(HSPA5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC343Mu01 | 70kDa熱休克蛋白5(HSPA5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPC343Mu61 | 70kDa熱休克蛋白5(HSPA5)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC343Mu03 | 70kDa熱休克蛋白5(HSPA5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC343Mu02 | 70kDa熱休克蛋白5(HSPA5)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAC343Mu01 | 70kDa熱休克蛋白5(HSPA5)多克隆抗體 | WB; IHC |
| ULAC343Mu71 | 70kDa熱休克蛋白5(HSPA5)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAC343Mu25 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | ICC/IF |
| UMAC343Mu22 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB |
| UMAC343Mu21 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB; IHC; ICC/IF |
| UMAC343Mu27 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB |
| UMAC343Mu26 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | ICC/IF |
| UMAC343Mu23 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB; IHC |
| UMAC343Mu24 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB; IHC |
| UFAC343Mu02 | 抗70kDa熱休克蛋白5(HSPA5)單克隆抗體 | FCM |
| USEC343Mu | 70kDa熱休克蛋白5(HSPA5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCC343Mu | 70kDa熱休克蛋白5(HSPA5)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMC343Mu | 70kDa熱休克蛋白5(HSPA5)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSC343Mu01 | 70kDa熱休克蛋白5(HSPA5)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| 41 | Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. [PubMed: 20522592] |
| pharmacological research | Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors [Pubmed: 31048034] |
| EPILEPSY & BEHAVIOR | Fenfluramine acts as a positive modulator of sigma-1 receptors [Pubmed: 32169824] |
| Journal of Oncology | Using Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer [33381181] |
| Heliyon | Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior [] |
| Medicine (Baltimore) | Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients [34414940] |
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