饑餓素(GHRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)
Mini Samples ELISA Kit for Ghrelin (GHRL)
MTLRP; Growth Hormone-Releasing Peptide
- 編號(hào)UMEA991Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍123.5-10,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于51.5pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1724 ¥ 2463 ¥ 11084 ¥ 20936 ¥ 172410
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)饑餓素(GHRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的饑餓素(GHRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 81-97 | 88 |
| heparin plasma(n=5) | 78-104 | 97 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的饑餓素(GHRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中饑餓素(GHRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-101% | 97-105% | 86-99% | 78-88% |
| EDTA plasma(n=5) | 78-104% | 85-92% | 78-91% | 93-101% |
| heparin plasma(n=5) | 82-101% | 80-98% | 88-95% | 81-101% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）25µL后，立即加入檢測(cè)溶液A25µL，
37°C孵育1小時(shí)；
3. 甩干，洗板3次；
4. 加檢測(cè)溶液B50µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分鐘；
7. 加終止液25µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競(jìng)爭(zhēng)抑制酶聯(lián)免疫分析法測(cè)定標(biāo)本中待測(cè)物質(zhì)水平。將饑餓素(GHRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測(cè)抗原(標(biāo)準(zhǔn)品或樣本)，待測(cè)抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競(jìng)爭(zhēng)結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過(guò)溫育和徹底洗滌后加入底物TMB顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測(cè)標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測(cè)物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（O.D.值），計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA991Ra11 | 饑餓素(GHRL)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA991Ra21 | 饑餓素(GHRL)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA991Ra08 | 饑餓素(GHRL)多克隆抗體 | WB; IHC; ICC; IP. |
| USEA991Ra | 饑餓素(GHRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA991Ra | 饑餓素(GHRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA991Ra | 饑餓素(GHRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA991Ra | 饑餓素(GHRL)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA991Ra11 | 饑餓素(GHRL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Journal of Physiology and Pharmacology | Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone [PubMed: 23211309] |
| Gene | The modifying effects of fish oil on fasting ghrelin mRNA expression in weaned rats [PubMed: 22842192] |
| Hormone Research in Paediatrics | Changes in Ghrelin and Obestatin Levels before and after a Meal in Children with Simple Obesity and Anorexia [Pubmed: 23774845] |
| Surgical Innovation | Gastric supply manipulation to modulate ghrelin production and enhance vascularization to the cardia: proof of the concept in a porcine model [Pubmed:25294792] |
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