單核細胞趨化蛋白1(MCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)
CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE
- 編號USEA087Si
- 物種Rhesus monkey (Simian，恒河猴) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.059ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1701 ¥ 2430 ¥ 10935 ¥ 20655 ¥ 170100
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測單核細胞趨化蛋白1(MCP1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的單核細胞趨化蛋白1(MCP1)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-101 | 95 |
| EDTA plasma(n=5) | 80-103 | 85 |
| heparin plasma(n=5) | 93-101 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的單核細胞趨化蛋白1(MCP1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中單核細胞趨化蛋白1(MCP1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-103% | 92-101% | 95-102% | 82-95% |
| EDTA plasma(n=5) | 96-104% | 87-99% | 89-97% | 96-104% |
| heparin plasma(n=5) | 95-104% | 94-102% | 95-102% | 81-97% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將單核細胞趨化蛋白1(MCP1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的單核細胞趨化蛋白1(MCP1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的單核細胞趨化蛋白1(MCP1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的單核細胞趨化蛋白1(MCP1)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rhesus monkey (Simian，恒河猴) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA087Si61 | 單核細胞趨化蛋白1(MCP1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA087Si61 | 單核細胞趨化蛋白1(MCP1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA087Si01 | 單核細胞趨化蛋白1(MCP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA087Si01 | 單核細胞趨化蛋白1(MCP1)多克隆抗體 | IHC |
| ULAA087Si71 | 單核細胞趨化蛋白1(MCP1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| ULAA087Si81 | 單核細胞趨化蛋白1(MCP1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UMAA087Si21 | 單核細胞趨化蛋白1(MCP1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA087Si | 單核細胞趨化蛋白1(MCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA087Si | 單核細胞趨化蛋白1(MCP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA087Si01 | 單核細胞趨化蛋白1(MCP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Cytokine | Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression. [Pubmed: 23434274] |
| Rheumatology International | Serum levels of selected chemokines in systemic lupus erythematosus patients [Pubmed: 22461186] |
| Food & Function | Effects of Hyriopsis cumingii polysaccharides on angiogenesis, macrophage chemotaxis, proliferation and phagocytosis [Pubmed:25620195] |
| 36 | MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cells [Pubmed:Pmc4176337] |
| Journal of Trace Elements in Medicine and Biology | Adipose tissue chromium and vanadium disbalance in high-fat fed Wistar rats [Pubmed:25194956] |
| 天津中醫(yī)藥大學學報 | 巨噬細胞及其相關(guān)因子在家兔不穩(wěn)定斑塊中的作用及其機制 [Paper:Source] |
| Int J Clin Exp Pathol | Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis [PubMed: 26191125] |
| Mediators of Inflammation | Effects of Intravenous Injection of [PubMed: 26063970] |
| Diabetes | Protein Inhibitor of Activated STAT 1 (PIAS1) Protects Against Obesity-Induced Insulin Resistance by Inhibiting Inflammation Cascade in Adipose Tissue [PubMed: 26324179] |
| Journal of Histochmistry and Cytochmistry | Epigallocatechin-3-Gallate Attenuates Unilateral Ureteral Obstruction-Induced Renal Interstitial Fibrosis in Mice [PubMed: 25549657] |
| Cell Physiol Biochem | MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1 [PubMed: 26159489] |
| Exp Biol Med (Maywood) | Renoprotective effects of angiotensin receptor blocker and stem cells in acute kidney injury: Involvement of inflammatory and apoptotic markers [PubMed: 25825359] |
| Parasitol Res | Trypanosoma cruzi infection and benznidazole therapy independently stimulate oxidative status and structural pathological remodeling of the liver tissue in mice [PubMed: 25912691] |
| Invest Ophthalmol Vis Sci | Simultaneous Increase in Multiple Proinflammatory Cytokines in the Aqueous Humor in Neovascular Glaucoma With and Without Intravitreal Bevacizumab Injection [PubMed: 26030108] |
| PLoS One | Postoperative changes in aqueous monocyte chemotactic protein-1 levels and bleb morphology after trabeculectomy vs. Ex-PRESS shunt surgery [PubMed: 26427058] |
| Veterinary Parasitology | Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140] |
| J Pharm Pharmacol | Modulation of oxidative and inflammatory cardiac response by nonselective 1‐and 3‐cyclooxygenase inhibitor and benznidazole in mice [PubMed: 26105112] |
| Микроэлементы в медицине | НАРУШЕНИЕ БАЛАНСА ХРОМА И ВАНАДИЯ В ЖИРОВОЙ ТКАНИ КАК ВОЗМОЖНЫЙ МЕХАНИЗМ ОЖИРЕНИЕ-АССОЦИИРОВАННОЙ ИНСУЛИНОРЕЗИСТЕНТНОСТИ [Microelements_Of_Midicine: 2015_2] |
| Front Pharmacol | The G-Protein-Coupled Bile Acid Receptor Gpbar1 (TGR5) Inhibits Gastric Inflammation Through Antagonizing NF-κB Signaling Pathway [PubMed: 26696888] |
| PLoS One | Effects of PB-EPCs on Homing Ability of Rabbit BMSCs via Endogenous SDF-1 and MCP-1. [PubMed: 26660527] |
| Scientific Reports | The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37Ra [Pubmed:26912204] |
| Neural Plasticity | Fractalkine Attenuates Microglial Cell Activation Induced by Prenatal Stress [Pubmed:27239349] |
| Technologia Alimentaria | Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats [abstract:10] |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602] |
| J Neurochem. | Anti‐inflammatory properties of tianeptine on lipopolysaccharide‐induced changes in microglial cells involve toll‐like receptor‐related pathways [Pubmed:26640965] |
| Oxidative Medicine and Cellular Longevity | Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation. [pubmed:27656261] |
| Comparative Clinical Pathology | Identification of some plasma biomarkers associated with early weaning stress in crossbred piglets [article:10.1007] |
| clinical chemistry and laboratory medicine | The serum concentrations of leptin and MCP-1 independently predict low back pain duration. [pubmed:28076310] |
| Journal of Molecular?Cell?Biology | oxLDL antibody inhibits MCP‐1 release in monocytes/macrophages by regulating Ca2+/K+ channel flow [pubmed:27995732] |
| Parasitology. | Nonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative/nitrosative stress and heart disease in T. cruzi-infected mice. [pubmed:28134069] |
| Experimental Parasitology | Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422] |
| National Natural Scientific | Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure [pubmed:29121396] |
| Evidence-Based Complementary and Alternative Medicine | BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway [journals:1758383] |
| International Journal of?Clinical?and Experimental Pathology | Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study [ISSN:1936-2625/IJCEP0055881] |
| Nature Medicine | An ALOX12-12-HETE-GPR31 signaling axis is a key mediator of hepatic ischemia-reperfusion injury. [pubmed:29227475] |
| Redox Biology | STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. [pubmed:29078168] |
| Nature Medicine | The deubiquitinating enzyme cylindromatosis mitigates nonalcoholic steatohepatitis [Pubmed:29291351] |
| NPG?Asia?Materials | Molybdenum disulfide/graphene oxide nanocomposites show favorable lung targeting and enhanced drug loading/tumor-killing efficacy with improved?… [Articles:am2017225] |
| Clinical?Science | Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity [Pubmed:29382697] |
| Life?Sciences | Lipoxin A4 may attenuate the progression of obesity-related glomerulopathy by inhibiting NF-κB and ERK/p38 MAPK-dependent inflammation [Pubmed:29499280] |
| Evidence-based?Complementary and Alternative?Medicine | Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling?… [Pubmed:29619063] |
| Lasers in Medical Science | Evaluation of the therapeutic effect of micro-plasma radio frequency on hypertrophic scars in rabbit ears [Pubmed:30003425] |
| Journal of Ocular Pharmacology and Therapeutics | Effect of 0.05% Difluprednate Ophthalmic Emulsion on Proinflammatory Cytokine Levels After Retinal Laser Photocoagulation in Rabbits [Pubmed:29812993] |
| Journal?of?Interferon?&?Cytokine?Research | Increased Serum MCP-1 Levels in Systemic Vasculitis Patients with Renal Involvement [Pubmed: 30230985] |
| Prostaglandins?&?Other?Lipid?Mediators | Detoxification and activating blood circulation decoction reduces restenosis involving the TLR4/NF-κB pathway after balloon injury [Pubmed: 30453089] |
| Molecular Neurobiology | Role of Chronic Administration of Antidepressant Drugs in the Prenatal Stress-Evoked Inflammatory Response in the Brain of Adult Offspring Rats?… [Pubmed: 30610610] |
| Journal?Of?Clinical?Investigation | Long non-coding RNA mitigates atherosclerosis by regulating the actin-binding protein NEXN [Pubmed: 30589415] |
| Kidney?International | Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome [Pubmed: 30770217] |
| Biosensors?and?Bioelectronics | A sensitive sandwich-type immunosensor for the detection of MCP-1 based on a rGO-TEPA-Thi-Au nanocomposite and novel RuPdPt trimetallic nanoalloy particles [] |
| European?Journal of Pharmacology | The cordycepin derivative IMM-H007 improves endothelial dysfunction by suppressing vascular inflammation and promoting AMPK-dependent eNOS activation in?… [Pubmed: 30826323] |
| Experimental?and?Therapeutic?Medicine | Effect of stress hyperglycaemia on monocyte chemoattractant protein?1 levels and the short?term prognosis of patients with acute ST?segment elevation myocardial?… [] |
| Hepatology | Integrated Omics Reveals Tollip as an Aggravator and Therapeutic Target for Hepatic Ischemia‐Reperfusion Injury in Mice [Pubmed: 31077413] |
| FASEB Journal | Rescuing cones and daylight vision in retinitis pigmentosa mice [Pubmed: 31199887] |
| Metallomics | Lanthanum chloride induces neuron damage by activating the nuclear factor-kappa B signaling pathway in activated microglia [Pubmed: 31187842] |
| Hepatology | Hepatocyte TRAF 6 Aggravates Hepatic Inflammation and Fibrosis by Promoting Lys6‐Linked Polyubiquitination of ASK 1 [] |
| journal of cellular and molecular medicine | LncRNA HOXA11‐AS regulates calcium oxalate crystal–induced renal inflammation via miR‐124‐3p/MCP‐1 [Pubmed: 31680444] |
| Diabetes | Histone H3 serine 10 phosphorylation facilitates endothelial activation in diabetic kidney disease [Pubmed: 30213824] |
| Biomedicine & Pharmacotherapy | Inhibition of CXCL1-CXCR2 axis ameliorates cisplatin-induced acute kidney injury by mediating inflammatory response [Pubmed: 31812015] |
| ACTA PHARMACOLOGICA SINICA | α-Galactosylceramide and its analog OCH differentially affect the pathogenesis of ISO-induced cardiac injury in mice [Pubmed: 32973325] |
| EBioMedicine | Modulating endothelial adhesion and migration impacts stem cell therapies efficacy [Pubmed: 32942121] |
| PHARMACOLOGICAL RESEARCH | Sea cucumbers-derived sterol sulfate alleviates insulin resistance and inflammation in high-fat-high-fructose diet-induced obese mice [Pubmed: 32911073] |
| BRITISH JOURNAL OF PHARMACOLOGY | A novel apoA‐I mimetic peptide suppresses atherosclerosis by promoting physiological HDL function in apoE?/? mice [Pubmed: 32726461] |
| Int Immunopharmacol | BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice [Pubmed: 32717566] |
| Life Sci | Impact of diminazene aceturate on renin-angiotensin system, infectious myocarditis and skeletal myositis in mice: An in vitro and in vivo study [Pubmed: 32652140] |
| Life Sciences | Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway. [Pubmed: 32603817] |
| Molecular Medicine Reports | Aquaporin?4 deletion ameliorates enterovirus 71 infection in mice [Pubmed: 32582978] |
| Life Sci | Protective function of exosomes from adipose tissue-derived mesenchymal stem cells in acute kidney injury through SIRT1 pathway [Pubmed: 32428599] |
| JOURNAL OF IMMUNOLOGY RESEARCH | β-Defensin Strengthens Antimicrobial Peritoneal Mast Cell Response [Pubmed: 32411798] |
| cell metabolism | Low-Dose Sorafenib Acts as a Mitochondrial Uncoupler and Ameliorates Nonalcoholic Steatohepatitis [Pubmed: 32375062] |
| Nanoscale Research Letters | Protective Effects of Carbon Dots Derived from Phellodendri Chinensis Cortex Carbonisata against Deinagkistrodon acutus Venom-Induced Acute Kidney?… [] |
| J Immunoassay Immunochem | Effects of regular exercise on inflammatory biomarkers and lipid parameters in soccer players [33750257] |
| FASEB Bioadv | Cyclophilin A induces macrophage apoptosis and enhances atherosclerotic lesions in high?\fat diet?\fed hyperglycemic rabbits [33977232] |
| Hepatology | TNIP3 is a novel activator of Hippo?\YAP signaling protecting against hepatic ischemia/reperfusion injury [34133792] |
| Bioactive Materials | Construction of heparin-based hydrogel incorporated with Cu5. 4O ultrasmall nanozymes for wound healing and inflammation inhibition [33778192] |
| researchsquare | p62 promotes HIV-1 gp120 V3 loop-mediated microglial inflammation by promoting noncanonical activation of Nrf2 [] |
| Transl Vis Sci Technol | Intraocular Pressure-Lowering Effects of Trabeculectomy Versus MicroShunt Insertion in Rabbit Eyes [34357381] |
| Cell Metabolism | Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species [34536344] |
| Stroke | CCL2 (CC Motif Chemokine Ligand 2) Biomarker Responses in Central Versus Peripheral Compartments After Focal Cerebral Ischemia [34587791] |
| J Ethnopharmacol | Identification of potential regulating effect of baicalin on NFκB/CCL2/CCR2 signaling pathway in rats with cerebral ischemia by antibody-based array and?… [34699947] |
| Biomed Pharmacother | Huangbai liniment and berberine promoted wound healing in high-fat diet/Streptozotocin-induced diabetic rats [Pubmed:35430394] |
| Expert Opinion on Therapeutic Targets | Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma [Pubmed:35549595] |
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