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胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Caspase 8 (CASP8)

CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog

  • 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 實驗結果圖
  • SEA853Mu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測胱天蛋白酶8(CASP8),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質(zhì)交叉反應檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實驗原理

將胱天蛋白酶8(CASP8)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的胱天蛋白酶8(CASP8)與連接于固相載體上的抗體結合,然后加入生物素化的胱天蛋白酶8(CASP8)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的胱天蛋白酶8(CASP8)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產(chǎn)品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
UEPA853Mu61 胱天蛋白酶8(CASP8)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPA853Mu61 胱天蛋白酶8(CASP8)活性蛋白 Cell?culture;?Activity?Assays.
URPA853Mu01 胱天蛋白酶8(CASP8)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPA853Mu02 胱天蛋白酶8(CASP8)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UEPA853Mu62 胱天蛋白酶8(CASP8)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAA853Mu01 胱天蛋白酶8(CASP8)多克隆抗體 WB; IHC
UPAA853Mu02 胱天蛋白酶8(CASP8)多克隆抗體 WB
ULAA853Mu81 胱天蛋白酶8(CASP8)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
ULAA853Mu71 胱天蛋白酶8(CASP8)多克隆抗體(生物素標記) WB
USEA853Mu 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA853Mu 胱天蛋白酶8(CASP8)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UKSA853Mu01 胱天蛋白酶8(CASP8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志 參考文獻
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Environ Toxicol Pharmacol. Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats [Pubmed: 31421311]
Journal of Biochemical and Molecular Toxicology Tranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferation [Pubmed: 32045101]
life sciences Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats [Pubmed: 32259601]
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337]
Int Immunopharmacol Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling [Pubmed: 33199237]
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways [Pubmed: 32942000]
Environ Sci Pollut Res Int Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low? dose of?… [Pubmed: 32915453]
J Biochem Mol Toxicol Tranilast abrogates cisplatin?\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation [34047436]
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