抗三碘甲狀腺原氨酸抗體(Anti-T3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Anti-Triiodothyronine Antibody (Anti-T3)
3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin
- 編號UAEA453Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法間接法
- 反應(yīng)時長2h, 30min
- 檢測范圍3.12-200ng/mL
- 靈敏度最小可檢測劑量小于等于1.27ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1785 ¥ 2550 ¥ 11475 ¥ 21675 ¥ 178500
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測抗三碘甲狀腺原氨酸抗體(Anti-T3)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的抗三碘甲狀腺原氨酸抗體(Anti-T3)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-94 | 81 |
| EDTA plasma(n=5) | 93-102 | 98 |
| heparin plasma(n=5) | 80-96 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的抗三碘甲狀腺原氨酸抗體(Anti-T3)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中抗三碘甲狀腺原氨酸抗體(Anti-T3)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-92% | 80-98% | 88-99% | 79-103% |
| EDTA plasma(n=5) | 96-105% | 88-102% | 88-97% | 78-105% |
| heparin plasma(n=5) | 92-103% | 94-105% | 78-97% | 95-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板5次；
5. 加TMB底物90µL，37°C孵育10-20分鐘；
6. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將抗原包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的抗三碘甲狀腺原氨酸抗體(Anti-T3)與連接于固相載體上的抗原結(jié)合，然后加入HRP標記的二抗，徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的抗三碘甲狀腺原氨酸抗體(Anti-T3)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA453Ge11 | 三碘甲狀腺原氨酸(T3)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA453Ge21 | 三碘甲狀腺原氨酸(T3)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA453Ge01 | 三碘甲狀腺原氨酸(T3)多克隆抗體 | ELISA |
| ULAA453Ge71 | 三碘甲狀腺原氨酸(T3)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA453Ge21 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA453Ge23 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA453Ge22 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA453Ge24 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA453Ge25 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA453Ge27 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | WB; IHC; ICC; IP. |
| ULAA453Ge72 | 三碘甲狀腺原氨酸(T3)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| UCEA453Ge | 三碘甲狀腺原氨酸(T3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA453Ge | 三碘甲狀腺原氨酸(T3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA453Ge | 三碘甲狀腺原氨酸(T3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA453Ge | 抗三碘甲狀腺原氨酸抗體(Anti-T3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| UCCA453Ge | 三碘甲狀腺原氨酸(T3)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA453Ge | 三碘甲狀腺原氨酸(T3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA453Ge11 | 三碘甲狀腺原氨酸(T3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Aquatic Toxicology | Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae [ScienceDirect: S0166445X12000215] |
| Aquatic Toxicology | Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis [PubMed: 23220413] |
| European Journal of Zoological Research | Effect of dietary tryptophan on plasma growth hormone and thyroid hormone in broiler chicks [Scholarsresearchlibrary: Source] |
| Life Science Journal | Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies [Lifesciencesite: Source] |
| PLoS One | A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism [Pubmed: 23468891] |
| Aquat Toxicol | Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay. [ScienceDirect: S0166445X13002129] |
| Aquatic Toxicology | Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae [Pubmed:25456233] |
| Chemosphere | Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam?Ruditapes philippinarum [science:S0045653516302016] |
| Toxicological?Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384] |
| Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267] |
| Aquat Toxicol. | Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus). [pubmed:28187359] |
| Bulletin of the World Health Organization | Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders [handle:123456789] |
| Biological Rhythm Research | Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes [09291016.2016.1275393] |
| Toxicological Sciences | Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction [pubmed:28973666] |
| Environmental Pollution | Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead [10.1016/j.envpol.2017.06.053] |
| Sciencepaper online | 四溴雙酚A對菲律賓蛤仔的生長抑制效應(yīng)機理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum [5790267088906950000] |
| Tropical Journal of Pharmaceutical Research | Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats [10.4314/tjpr.v16i5.9] |
| s-space | Screening thyroid hormone disrupting effects of benzophenones using GH3, FRTL-5 cells and zebrafish [10371/137708] |
| s-space | Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult [10371/137708] |
| Environmental Pollution | Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish. [pubmed:28624628] |
| Environmental?Science?& Technology | Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafish [Pubmed:29995391] |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625] |
| Toxicological?Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci] |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
| Drug?metabolism and disposition | Activation of 5-HT1A receptors in the hypothalamic paraventricular nuclei (PVN) negatively regulates cytochrome P450 expression and activity in rat liver [Pubmed:29555828] |
| Archives?Animal?Breeding | Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes [10.5194:aab-61-191-2018] |
| Indian Journal of Animal Sciences | Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition [:] |
| Environmental Pollution | Transgenerational thyroid endocrine disruption induced by bisphenol S affects the early development of zebrafish offspring [Pubmed: 30243188] |
| Cell?Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008] |
| Drug Metabolism and Disposition | Serotonin receptors of 5-HT2 type in the hypothalamic arcuate nuclei (ARC) positively regulate liver cytochrome P450 via stimulation of the growth hormone-releasing?… [Pubmed: 30518657] |
| Science?of?the?Total?Environment | Parental exposure to 6: 2 chlorinated polyfluorinated ether sulfonate (F53B) induced transgenerational thyroid hormone disruption in zebrafish [Doi: 10.1016/j.scitotenv.2019.02.198] |
| Theriogenology? | Thyroid hormone profiles and TSH evaluation during early pregnancy and the transition period in dairy cows [Pubmed: 30784791] |
| Chemosphere | Inhibition of growth in juvenile manila clam Ruditapes philippinarum: Potential adverse outcome pathway of TBBPA [Pubmed: 30844590] |
| Aquatic Toxicology | Effects of cis-bifenthrin enantiomers on the growth, behavioral, biomarkers of oxidative damage and bioaccumulation in Xenopus laevis [Pubmed: 31276910] |
| ecotoxicology and environmental safety | Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvae [Pubmed: 31539807] |
| Cells | MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model [Pubmed: 31540304] |
| Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938] |
| Autoimmunity | Therapeutic effect of mesenchymal stem cell on Hashimoto's thyroiditis in a rat model by modulating Th17/Treg cell balance [Pubmed: 31793369] |
| AQUATIC TOXICOLOGY | Interaction between hypoxia and perfluorobutane sulfonate on developmental toxicity and endocrine disruption in marine medaka embryos [Pubmed: 32172180] |
| Chemosphere | Effect of triadimefon and its metabolite on adult amphibians Xenopus laevis [Pubmed: 31743868] |
| Nature | Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis [Pubmed: 33177714] |
| Toxicol Lett | Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio) [Pubmed: 32525014] |
| Ecotoxicology | Changes in thyroid hormone levels and related gene expressions in embryo–larval zebraish exposed to binary combinations of bifenthrin and acetochlor [Pubmed: 32468518] |
| Brazilian Journal of Development | Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposi??o materna ao triclosan causa?… [] |
| CHEMOSPHERE | Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio) [] |
| Andrologia | Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine?\treated rat dams during pregnancy and lactation [33433934] |
| The Journal of Clinical Endocrinology & Metabolism | High levels of thyroid hormone impair regulatory T cells function via reduced PD-1 expression [33758937] |
| Endocrinology | Genetic Manipulation on Zebrafish duox Recapitulate the Clinical Manifestations of Congenital Hypothyroidism [34019632] |
| Ecotoxicol Environ Saf | Xenopus laevis tadpoles exposed to metamifop: Changes in growth, behavioral endpoints, neurotransmitters, antioxidant system and thyroid development [34126306] |
| Journal of Hazardous Materials | Exposure of adult zebrafish (Danio rerio) to Tetrabromobisphenol A causes neurotoxicity in larval offspring, an adverse transgenerational effect [33647619] |
| Pharmaceutics | Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays [] |
| Pharmaceutics | The Selective NMDA Receptor GluN2B Subunit Antagonist CP-101,606 with Antidepressant Properties Modulates Cytochrome P450 Expression in the Liver [34683936] |
| Aquat Toxicol | Thyroid disruption and growth inhibition of zebrafish embryos/larvae by phenanthrene treatment at environmentally relevant concentrations [34933138] |
| Environmental Pollution | Probiotics inhibit the stunted growth defect of perfluorobutanesulfonate via stress and thyroid axes in zebrafish larvae [34428700] |
| Ecotoxicol Environ Saf | Perfluorohexanoic acid caused disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae [Pubmed:35131581] |
| Toxics | Environmental Co-Exposure to Potassium Perchlorate and Cd Caused Toxicity and Thyroid Endocrine Disruption in Zebrafish Embryos and Larvae (Danio rerio) [Pubmed:35448459] |
留言咨詢
