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波形蛋白(VIM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Vimentin (VIM)

  • 波形蛋白(VIM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 波形蛋白(VIM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對波形蛋白(VIM)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
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USEB040Hu 波形蛋白(VIM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
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UKSB040Hu01 波形蛋白(VIM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Cancer?Epidemiology,?Biomarkers?& Prevention Aberrant Vimentin Methylation Is Characteristic of Breast Cancer [pubmed:22315367]
Scientific Reports Glyoxalase?1-knockdown?in?human?aortic?endothelial?cells - effect on the?proteome?andendothelial?function?estimates. [pubmed:27898103]
Journal of?Proteomics HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin [Pubmed:29477555]
Journal of Biological Chemistry Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328]
American Journal of Transplantation Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts [Pubmed: 31729155]
Lung Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? [Pubmed: 32088750]
Cell Biol Toxicol ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer [33566221]
CELL BIOLOGY AND TOXICOLOGY Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence [33758996]
Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women []
Advanced glycation end-products associate with podocytopathy in type II diabetic patients []
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… []
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