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多配體蛋白聚糖1(SDC1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Syndecan 1 (SDC1)

CD138; SDC; SYND1; Syndecan Proteoglycan 1

  • 多配體蛋白聚糖1(SDC1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 多配體蛋白聚糖1(SDC1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對多配體蛋白聚糖1(SDC1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPB966Mu02 多配體蛋白聚糖1(SDC1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPB966Mu01 多配體蛋白聚糖1(SDC1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UEPB966Mu62 多配體蛋白聚糖1(SDC1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAB966Mu01 多配體蛋白聚糖1(SDC1)多克隆抗體 WB; IHC
UPAB966Mu02 多配體蛋白聚糖1(SDC1)多克隆抗體 WB; IHC; ICC; IP.
USEB966Mu 多配體蛋白聚糖1(SDC1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB966Mu 多配體蛋白聚糖1(SDC1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UKSB966Mu01 多配體蛋白聚糖1(SDC1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Molecular and Cellular Biochemistry Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction [SpringerLink: a201w3l18k257462]
Inflammation Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats [Springer: Source]
Atherosclerosis. Damage of the endothelial glycocalyx in chronic kidney disease [Pubmed:24727235]
Journal of Trauma and Acute Care Surgery Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury [Pubmed:25058256]
Shock Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance [Pubmed:25692257]
Mediators Inflamm Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding [PubMed: 26199464]
Transplantation Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury. [PubMed: 26371596]
Clinical and Experimental Nephrology Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury [pubmed:27405618]
Biomarkers in Medicine Endothelial glycocalyx layer shedding following lung resection [pubmed:27643669]
Danish National RESEARCH DATABASE Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats [10.1089]
Journal of Neurotrauma Resuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat. [doi/10.1089/neu.2016.4574]
British Journal of Anaesthesia Volume kinetics of Ringer's lactate solution in acute inflammatory disease [10.1016:j.bja.2018.04.023]
Arteriosclerosis, Thrombosis, and?Vascular Biology MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic charge [Pubmed:29903730]
Shock Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsis [Pubmed:28591008]
Shock Experimental models of endotheliopathy: impact of shock severity [Pubmed:28697004]
Journal of?Trauma?and?Acute?Care?Surgery Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the rat [Pubmed:28930945]
The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions [173015_06.pdf]
Journal of Surgical Research Plasma ameliorates endothelial dysfunction in burn injury [Doi: 10.1016/j.jss.2018.08.027]
Journal of Anesthesia Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model [Pubmed: 30374889]
Anaesthesia Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass [Pubmed: 30687934]
Journal of Cardiothoracic and Vascular Anesthesial Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuit [Pubmed: 31787433]
Hepatobiliary & Pancreatic Diseases International Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICP [Pubmed: 31919038]
Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study []
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis [33375342]
Microvasc Res Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency [34119534]
J Gerontol A Biol Sci Med Sci The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo?… [34423832]
Shock Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke [34524269]
Exp Anim Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock model [Pubmed:35110424]
Journal of Emergency Medicine Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase []
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