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分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Secreted Frizzled Related Protein 1 (SFRP1)

FRP1; FRP; FRP-1; FrzA; SARP2; Secreted apoptosis-related protein 2

  • 分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對分泌型卷曲相關(guān)蛋白1(SFRP1)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Mus musculus (Mouse,小鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
URPF880Mu02 分泌型卷曲相關(guān)蛋白1(SFRP1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPF880Mu01 分泌型卷曲相關(guān)蛋白1(SFRP1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAF880Mu01 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體 WB; IHC
UPAF880Mu02 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體 WB; IHC; ICC; IP.
ULAF880Mu71 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體(生物素標記) WB; IHC; ICC.
ULAF880Mu81 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
USEF880Mu 分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMF880Mu 分泌型卷曲相關(guān)蛋白1(SFRP1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UKSF880Mu01 分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志 參考文獻
BMC Musculoskeletal Disorders Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis [PubMed: 23025387]
Exp Cell Res Wnt Inhibition is Dysregulated in Gliomas and its Re-Establishment Inhibits Proliferation and Tumor Sphere Formation [PubMed: 26712519]
BMC Musculoskeletal Disorders Correlation?of?blood?bone?turnover?biomarkers?and?Wnt?signaling?antagonists?with AS,?DISH,OPLL, and?OYL. [pubmed:28153008]
PloS one Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial. [pubmed:28178355]
PLoS One Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial [10.1371]
Journal of Experimental & Clinical Cancer Research PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway [pubmed:28645312]
JBMR Plus Low serum levels of DKK2 predict incident low‐impact fracture in older women [Doi: 10.1002/jbm4.10179]
Nature Communications Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway [Pubmed: 32041953]
acta pharmacologica sinica sFRP1 protects H9c2 cardiac myoblasts from doxorubicin-induced apoptosis by inhibiting the Wnt/PCP-JNK pathway [Pubmed: 32238888]
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