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凝血酶原片段F1+2(F1+2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Prothrombin Fragment 1+2 (F1+2)

  • 凝血酶原片段F1+2(F1+2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 凝血酶原片段F1+2(F1+2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對凝血酶原片段F1+2(F1+2)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
URPA710Hu01 凝血酶原片段F1+2(F1+2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAA710Hu01 凝血酶原片段F1+2(F1+2)多克隆抗體 WB; IHC; ICC/IF
ULAA710Hu71 凝血酶原片段F1+2(F1+2)多克隆抗體(生物素標記) WB; IHC; ICC.
UMAA710Hu22 凝血酶原片段F1+2(F1+2)單克隆抗體 WB; IHC
UMAA710Hu23 凝血酶原片段F1+2(F1+2)單克隆抗體 WB; IHC; ICC/IF
UMAA710Hu24 凝血酶原片段F1+2(F1+2)單克隆抗體 WB; IHC
UMAA710Hu25 凝血酶原片段F1+2(F1+2)單克隆抗體 WB; ICC/IF
UMAA710Hu21 凝血酶原片段F1+2(F1+2)單克隆抗體 IHC
UMAA710Hu26 凝血酶原片段F1+2(F1+2)單克隆抗體 WB
UFAA710Hu02 抗凝血酶原片段F1+2(F1+2)單克隆抗體 FCM
ULAA710Hu72 凝血酶原片段F1+2(F1+2)單克隆抗體(生物素標記) WB; IHC; ICC.
USEA710Hu 凝血酶原片段F1+2(F1+2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
UHEA710Hu 凝血酶原片段F1+2(F1+2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
USCA710Hu 凝血酶原片段F1+2(F1+2)檢測試劑盒(化學發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
ULMA710Hu 凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UPSA710Hu01 凝血酶原片段F1+2(F1+2)抗體對 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
UKSA710Hu01 凝血酶原片段F1+2(F1+2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志 參考文獻
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Journal of Vascular Surgery Changes in thrombin generation, fibrinolysis, platelet and endothelial cell activity, and inflammation following endovascular abdominal aortic aneurysm repair [ScienceDirect: S0741521411018568]
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine High levels of soluble VEGF receptor 1 early after trauma are associated with shock, sympathoadrenal activation, glycocalyx degradation and inflammation in severely injured patients: a prospective study [Sjtrem:1757-7241]
Journal of Thrombosis and Haemostasis High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality [Wiley: source]
British Journal of Dermatology Evaluation of autologous plasma skin test in patients with chronic idiopathic urticaria [PubMed: 21910697]
Arteriosclerosis, Thrombosis, and Vascular Biology. Diet Modulates Endogenous Thrombin Generation, A Biological Estimate of Thrombosis Risk, Independently of the Metabolic Status [PubMed: 22859493]
Journal of Vascular Surgery Medium-term effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis [PubMed: 23140799]
PLoS ONE Markers of Thrombogenesis and Fibrinolysis and Their Relation to Inflammation and Endothelial Activation in Patients with Idiopathic Pulmonary Arterial Hypertension [Plosone: Source]
Journal of Vascular Surgery Effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis [Pubmed: 23140799 ]
Haemetology Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India [Pubmed: 23992124]
Cogent Medicine Study the association of adiponectin with inflammation and hypercoagulability in case of type 2 diabetic subjects with renal dysfunction [Tandfonline:Source]
Interact Cardiovasc Thorac Surg. An ex vivo evaluation of blood coagulation and thromboresistance of two extracorporeal circuit coatings with reduced and full heparin dose [Pubmed:24632424]
Perfusion Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing [PubMed: 26243277]
J Thromb Haemost Comparison of the Effect of Dabigatran and Dalteparin on Thrombus Stability in a Murine Model of Venous Thromboembolism [PubMed: 26514101]
Acta Oncologica Turcica Thrombin activatable fibrinolysis inhibitor (TAFI), tissue factor pathway inhibitor (TFPI), and prothrombin fragment 1+2 levels in patients with advanced colorectal cancer [aot:AOT_49_1_6_12.pdf]
Advances in Medical Sciences Perioperative thrombocytopenia predicts poor outcome in patients undergoing transcatheter aortic valve implantation [pubmed:29145170]
Thrombosis Research The causes of thrombocytopenia after transcatheter aortic valve implantation [pubmed:28582640]
Acta?Haematologica Elevations of Thrombotic Biomarkers in Hemoglobin H Disease [Pubmed:29402840]
Journal of Neurotrauma The cerebral thrombin system is activated after intracerebral hemorrhage and contributes to secondary lesion growth and poor neurological outcome in C57Bl/6 mice [Pubmed: 31830857]
Indian Journal of Public Health Research & Development Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever []
Rheumatol Int The impact of anti-endothelial cell antibodies (AECAs) on the development of blood vessel damage in patients with systemic lupus erythematosus: the preliminary?… [Pubmed:35284968]
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