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甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Parathyroid Hormone Related Protein (PTHrP)

PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone

  • 甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對甲狀旁腺激素相關蛋白(PTHrP)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
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參考文獻

雜志 參考文獻
Clinical and Experimental Metastasis Characterization of a new renal cell carcinoma bone metastasis mouse model [PubMed: 20443133]
Stem Cells and Development Temporal activation of β-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated. [PubMed: 22133004]
International journal of biological sciences PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast Inhibition [PubMed: PMC3753447]
Investigational New Drugs Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclasts [Pubmed: 23673814]
Toxicology and Applied Pharmacology Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatment [Pubmed:24463094]
Arch Gynecol Obstet. Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study [Pubmed:24943060]
PLoS One 15-Deoxy-Δ12, 14-Prostaglandin J2 Inhibits Osteolytic Breast Cancer Bone Metastasis and Estrogen Deficiency-Induced Bone Loss [PubMed: 25859665]
Endocrinology A role for the calcitonin receptor to limit bone loss during lactation in female mice by inhibiting osteocytic osteolysis [PubMed: 26135836]
Tumour Biol Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation. [Pubmed:27312487]
35 Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectin [pubmed:27740628]
Bone Lactation induces increases in the RANK/RANKL/OPG system in maxillary bone [Pubmed:29382610]
International Journal of Molecular Sciences A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic Mice [Pubmed: 31600911]
Journal of Orthopaedic Surgery and Research Identification of a potential gene target for osteoarthritis based on bioinformatics analyses [Pubmed: 32571421]
International Immunopharmacology Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related?… []
Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни []
Cell Death Dis Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ?- [33510128]
Sci Rep Effects of whole-body vibration training in a cachectic C26 mouse model [34732809]
Acta Physiologica The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals [Pubmed:35334154]
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