骨粘連蛋白(ON)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Osteonectin (ON)
SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine
- 編號UKSA791Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍3.12-200ng/mL
- 可適靈敏度1.42ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 3402 ¥ 5670 ¥ 10206 ¥ 19845 ¥ 34020
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對骨粘連蛋白(ON)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| UAPA791Hu01 | 骨粘連蛋白(ON)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA791Hu61 | 骨粘連蛋白(ON)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA791Hu61 | 骨粘連蛋白(ON)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA791Hu01 | 骨粘連蛋白(ON)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPA791Hu21 | 骨粘連蛋白(ON)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA791Hu01 | 骨粘連蛋白(ON)多克隆抗體 | WB |
| UPAA791Hu08 | 骨粘連蛋白(ON)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA791Hu81 | 骨粘連蛋白(ON)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA791Hu71 | 骨粘連蛋白(ON)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA791Hu22 | 骨粘連蛋白(ON)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA791Hu | 骨粘連蛋白(ON)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA791Hu | 骨粘連蛋白(ON)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA791Hu01 | 骨粘連蛋白(ON)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| J Mol Med (Berl) | Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistance [PubMed: 26199111] |
| Muscle Nerve | Effect of resistance ladder training on SPARC expression in skeletal muscle of hindlimb immobilized rats [PubMed: 26467437] |
| Medicine (Baltimore) | An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370] |
| British Journal of Cancer | A proteomics-based approach identifies secreted protein acidic and rich in cysteine as a prognostic biomarker in malignant pleural mesothelioma [Pubmed:26889976] |
| Journal of diabetes and its complications | Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. [pubmed:27916484] |
| American journal of the medical sciences | Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus [pubmed:29078839] |
| Translational Research in Oral Oncology | Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancer [Doi: 10.1177/2057178X18800534] |
| [ENGINEERING OF BIOMATERIALS | Osteoconductive potential of PLGA/bioglass composite biomaterials [] |
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