胰高血糖素(GCG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Glucagon (GCG)
GC; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone
- 編號UKSB266Ra11
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競爭物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍18.52-1500pg/mL
- 可適靈敏度8.86pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 3899 ¥ 6498 ¥ 11696 ¥ 22743 ¥ 38988
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對胰高血糖素(GC)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB266Ra01 | 胰高血糖素(GCG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB266Ra01 | 胰高血糖素(GCG)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAB266Ra21 | 胰高血糖素(GCG)單克隆抗體 | WB; IHC; ICC; IP. |
| UCEB266Ra | 胰高血糖素(GCG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB266Ra | 胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB266Ra11 | 胰高血糖素(GCG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Molecular Metabolism | PER2 promotes glucose storage to liver glycogen during feeding and acute fasting by inducing Gys2 PTG and GL expression [PubMed: PMC3773838] |
| Exp Biol Med (Maywood). | Increasing glucagon secretion could antagonize the action of exogenous insulin for glycemic control in streptozocin-induced diabetic rhesus monkeys. [Pubmed: 23760004] |
| European Journal of Endocrinology | Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in human [Eje-online: Source] |
| American Diabetes Association | IL-21 is a major negative regulator of IRF4-dependent lipolysis affecting Tregs in adipose tissue and systemic insulin sensitivity [Pubmed:24430438] |
| Journal of Pharmacy and Pharmacology | Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db?mice, a model of type 2 diabetes mellitus [Pubmed:24611864] |
| European Journal of Pharmacology | Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model [Pubmed:27131827] |
| Appl Physiol Nutr Metab | The glucoreCavia (Guinea pig )latory response to high-intensity aerobic exercise following training in rats with insulin-treated type 1 diabetes mellitus [Pubmed:27175938] |
| American Journal of Translational Research | Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β. [pubmed:28337257] |
| Biomedicine & Pharmacotherapy | Vitamin?D3?intake?as?regulator?of?insulin?degrading?enzyme?and?insulin?receptor?phosphorylationin?diabetic?rats. [pubmed:27930980] |
| Diabetologia | Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice [pubmed:28280902] |
| International?Journal?of?Molecular?Sciences | Effects of Acute Cold Stress on Liver O-GlcNAcylation and Glycometabolism in Mice [Pubmed: 30231545] |
| European?Journal?of?Pharmacology | Exogenous obestatin decreases beta-cell apoptosis and alfa-cell proliferation in high fat diet and streptozotocin induced type 2 diabetic rats [Pubmed: 30776368] |
| Stress | Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure [Pubmed: 30821572] |
| international?journal?of?molecular?sciences | Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice [Pubmed: 30909542] |
| Diabetes-Metabolism Research and Reviews | Liraglutide Combined with Human Umbilical Cord Mesenchymal Stem Cell Transplantation inhibits beta‐cells apoptosis via mediating the ASK1/JNK/BAX pathway in [Pubmed: 31411368] |
| CELL DISCOVERY | In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals [Pubmed: 32284878] |
| endocrine journal | Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose?… [Pubmed: 32249244] |
| Frontiers in Endocrinology | RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034] |
| Molecular Medicine Reports | Acarbose ameliorates spontaneous type?2 diabetes in db/db mice by inhibiting PDX?1 methylation [Pubmed: 33236139] |
| CHEMICO-BIOLOGICAL INTERACTIONS | Modulatory role of imatinib mesylate on pancreatic β-cells' secretory functions in an STZ rat model of diabetes mellitus [Pubmed: 32710900] |
| Mol Cell Endocrinol | Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells [34391847] |
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