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胰高血糖素(GCG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Glucagon (GCG)

GC; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone

  • 胰高血糖素(GCG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產品包裝(模擬)
  • 胰高血糖素(GCG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

試劑盒中的抗體對胰高血糖素(GC)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

儲存

抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。

配套試劑

相關產品

編號 適用物種：Mus musculus (Mouse，小鼠) 應用(僅供研究使用，不用于臨床診斷！)
URPB266Mu01 胰高血糖素(GCG)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPB266Mu02 胰高血糖素(GCG)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAB266Mu02 胰高血糖素(GCG)多克隆抗體 WB; IHC; ICC; IP.
UPAB266Mu01 胰高血糖素(GCG)多克隆抗體 WB; IHC
UCEB266Mu 胰高血糖素(GCG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB266Mu 胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UKSB266Mu11 胰高血糖素(GCG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Molecular Metabolism PER2 promotes glucose storage to liver glycogen during feeding and acute fasting by inducing Gys2 PTG and GL expression [PubMed: PMC3773838]
Exp Biol Med (Maywood). Increasing glucagon secretion could antagonize the action of exogenous insulin for glycemic control in streptozocin-induced diabetic rhesus monkeys. [Pubmed: 23760004]
European Journal of Endocrinology Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in human [Eje-online: Source]
American Diabetes Association IL-21 is a major negative regulator of IRF4-dependent lipolysis affecting Tregs in adipose tissue and systemic insulin sensitivity [Pubmed:24430438]
Journal of Pharmacy and Pharmacology Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db?mice, a model of type 2 diabetes mellitus [Pubmed:24611864]
European Journal of Pharmacology Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model [Pubmed:27131827]
Appl Physiol Nutr Metab The glucoreCavia (Guinea pig )latory response to high-intensity aerobic exercise following training in rats with insulin-treated type 1 diabetes mellitus [Pubmed:27175938]
American Journal of Translational Research Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β. [pubmed:28337257]
Biomedicine & Pharmacotherapy Vitamin?D3?intake?as?regulator?of?insulin?degrading?enzyme?and?insulin?receptor?phosphorylationin?diabetic?rats. [pubmed:27930980]
Diabetologia Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice [pubmed:28280902]
International?Journal?of?Molecular?Sciences Effects of Acute Cold Stress on Liver O-GlcNAcylation and Glycometabolism in Mice [Pubmed: 30231545]
European?Journal?of?Pharmacology Exogenous obestatin decreases beta-cell apoptosis and alfa-cell proliferation in high fat diet and streptozotocin induced type 2 diabetic rats [Pubmed: 30776368]
Stress Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure [Pubmed: 30821572]
international?journal?of?molecular?sciences Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice [Pubmed: 30909542]
Diabetes-Metabolism Research and Reviews Liraglutide Combined with Human Umbilical Cord Mesenchymal Stem Cell Transplantation inhibits beta‐cells apoptosis via mediating the ASK1/JNK/BAX pathway in [Pubmed: 31411368]
CELL DISCOVERY In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals [Pubmed: 32284878]
endocrine journal Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose?… [Pubmed: 32249244]
Frontiers in Endocrinology RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034]
Molecular Medicine Reports Acarbose ameliorates spontaneous type?2 diabetes in db/db mice by inhibiting PDX?1 methylation [Pubmed: 33236139]
CHEMICO-BIOLOGICAL INTERACTIONS Modulatory role of imatinib mesylate on pancreatic β-cells' secretory functions in an STZ rat model of diabetes mellitus [Pubmed: 32710900]
Mol Cell Endocrinol Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells [34391847]
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