成纖維細胞生長因子15(FGF15)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Fibroblast Growth Factor 15 (FGF15)
- 編號UKSL154Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.070ng/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 4180 ¥ 6966 ¥ 12539 ¥ 24381 ¥ 41796
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對成纖維細胞生長因子15(FGF15)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| UAPL154Ra01 | 成纖維細胞生長因子15(FGF15)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPL154Ra01 | 成纖維細胞生長因子15(FGF15)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAL154Ra01 | 成纖維細胞生長因子15(FGF15)多克隆抗體 | WB; IHC |
| UMAL154Ra22 | 成纖維細胞生長因子15(FGF15)單克隆抗體 | IHC |
| UMAL154Ra23 | 成纖維細胞生長因子15(FGF15)單克隆抗體 | IHC |
| UMAL154Ra24 | 成纖維細胞生長因子15(FGF15)單克隆抗體 | IHC |
| UMAL154Ra25 | 成纖維細胞生長因子15(FGF15)單克隆抗體 | IHC |
| UMAL154Ra27 | 成纖維細胞生長因子15(FGF15)單克隆抗體 | IHC |
| UMAL154Ra21 | 成纖維細胞生長因子15(FGF15)單克隆抗體 | IHC |
| UWEL154Ra | 成纖維細胞生長因子15(FGF15)檢測試劑盒(酶聯免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEL154Ra | 成纖維細胞生長因子15(FGF15)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEL154Ra | 成纖維細胞生長因子15(FGF15)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULML154Ra | 成纖維細胞生長因子15(FGF15)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSL154Ra01 | 成纖維細胞生長因子15(FGF15)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Endocrinology. | Naringenin Prevents Obesity, Hepatic Steatosis, and Glucose Intolerance in Male Mice Independent of Fibroblast Growth Factor 21 [PubMed: 25774553] |
| Inflammatory Bowel Diseases | Alterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammation [pubmed:27580383] |
| J Lipid Res. | Commensal bacteria at the crossroad between cholesterol homeostasis and chronic inflammation in atherosclerosis. [pubmed:28130274] |
| BMC Endocrine Disorders | The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance [10.1186/s12902-017-0211-5] |
| Journal?of?Physiology?and?Biochemistry | The ileal FGF15/19 to hepatic FGFR4 axis regulates liver regeneration after partial hepatectomy in mice [Pubmed:29468415] |
| Developmental?Cell | FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis [Pubmed: 30745141] |
| Endocrinology | Interactions between the gravitostat and the fibroblast growth factor system for the regulation of body weight [] |
| Food & Function | Lactoferrin promotes bile acid metabolism and reduces hepatic cholesterol deposition by inhibiting the farnesoid X receptor (FXR)-mediated enterohepatic axis [Pubmed: 31626262] |
| Journal of Translational Medicine | Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation [Pubmed: 33036625] |
| Chin Med | Si-Wu-Tang ameliorates fibrotic liver injury via modulating intestinal microbiota and bile acid homeostasis [34736501] |
| Int J Biol Sci | Dynamics of the gut-liver axis in rats with varying fibrosis severity [Pubmed:35637968] |
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