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上皮中性粒細(xì)胞激活肽78(ENA78)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Epithelial Neutrophil Activating Peptide 78 (ENA78)

CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys),Member 5; Neutrophil-activating peptide ENA-78

  • 上皮中性粒細(xì)胞激活肽78(ENA78)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 上皮中性粒細(xì)胞激活肽78(ENA78)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對上皮中性粒細(xì)胞激活肽78(ENA78)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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編號 適用物種:Mus musculus (Mouse,小鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
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UAPA860Mu61 上皮中性粒細(xì)胞激活肽78(ENA78)活性蛋白 Cell?culture;?Activity?Assays.
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UPAA860Mu01 上皮中性粒細(xì)胞激活肽78(ENA78)多克隆抗體 WB; IHC; ICC; IP.
ULAA860Mu81 上皮中性粒細(xì)胞激活肽78(ENA78)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
ULAA860Mu71 上皮中性粒細(xì)胞激活肽78(ENA78)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
USEA860Mu 上皮中性粒細(xì)胞激活肽78(ENA78)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
UAEA860Mu 抗上皮中性粒細(xì)胞激活肽78抗體(Anti-ENA78)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antibody Detection.
USCA860Mu 上皮中性粒細(xì)胞激活肽78(ENA78)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
ULMA860Mu 上皮中性粒細(xì)胞激活肽78(ENA78)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UKSA860Mu01 上皮中性粒細(xì)胞激活肽78(ENA78)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 自己動手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of Biochemical and Molecular Toxicology Ameliorative Effects of Curcumin on Fibrinogen-Like Protein-2 Gene Expression, Some Oxido-Inflammatory and Apoptotic Markers in a Rat Model of l-Arginine-Induced Acute Pancreatitis. [Pubmed:26862043]
Journal of Biochemical and Molecular Toxicology Ameliorative Effects of Curcumin on Fibrinogen-Like Protein-2 Gene Expression, Some Oxido-Inflammatory and Apoptotic Markers in a Rat Model of l-Arginine-Induced Acute Pancreatitis [pubmed:26862043]
OncoTargets and Therapy The clinical significance of CXCL5 in non-small cell lung cancer. [pubmed:29200871]
Biochimie Activated CXCL5-CXCR2 axis promotes the migration, invasion and EMT of papillary thyroid carcinoma cells via modulation of β-catenin pathway [Pubmed:29471001]
Cancer?Biology?&?Therapy Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38?… [Pubmed: 30404567]
Respiratory?Research Phospholipase Cε plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from?… [Pubmed: 30634975]
American Journal of Pathology Interleukin17–CXCR2 axis facilitates breast cancer progression by up-regulating neutrophil recruitment [Pubmed: 31654638]
Bioactive Materials Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy [34820587]
Am J Cancer Res Differential expression profile of CXC-receptor-2 ligands as potential biomarkers in pancreatic ductal adenocarcinoma [Pubmed:35141005]
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