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多配體蛋白聚糖1(SDC1)檢測試劑盒(化學發(fā)光免疫分析法)

CLIA Kit for Syndecan 1 (SDC1)

CD138; SDC; SYND1; Syndecan Proteoglycan 1

  • 多配體蛋白聚糖1(SDC1)檢測試劑盒(化學發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 多配體蛋白聚糖1(SDC1)檢測試劑盒(化學發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 多配體蛋白聚糖1(SDC1)檢測試劑盒(化學發(fā)光免疫分析法) 實驗結果圖
  • SCB966Hu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測多配體蛋白聚糖1(SDC1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的多配體蛋白聚糖1(SDC1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 81-102 95

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的多配體蛋白聚糖1(SDC1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中多配體蛋白聚糖1(SDC1)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 97-105% 81-93% 80-99%
EDTA plasma(n=5) 91-101% 87-96% 84-105% 89-98%
heparin plasma(n=5) 98-105% 89-105% 82-102% 79-101%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。

實驗原理

將多配體蛋白聚糖1(SDC1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的多配體蛋白聚糖1(SDC1)與連接于固相載體上的抗體結合，然后加入生物素化的多配體蛋白聚糖1(SDC1)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的多配體蛋白聚糖1(SDC1)呈正相關。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。

相關產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
UEPB966Hu62 多配體蛋白聚糖1(SDC1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPB966Hu01 多配體蛋白聚糖1(SDC1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UEPB966Hu61 多配體蛋白聚糖1(SDC1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPB966Hu02 多配體蛋白聚糖1(SDC1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPB966Hu03 多配體蛋白聚糖1(SDC1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAB966Hu01 多配體蛋白聚糖1(SDC1)多克隆抗體 WB; IHC; ICC/IF
UFAB966Hu01 抗多配體蛋白聚糖1(SDC1)多克隆抗體 FCM
ULAB966Hu81 多配體蛋白聚糖1(SDC1)多克隆抗體(異硫氰酸熒光素標記) WB
UFAB966Hu41 抗多配體蛋白聚糖1(SDC1)多克隆抗體(藻紅素標記) Flow cytometry.
UFAB966Hu51 抗多配體蛋白聚糖1(SDC1)多克隆抗體(別藻藍蛋白標記) Flow cytometry.
UFAB966Hu81 抗多配體蛋白聚糖1(SDC1)多克隆抗體(異硫氰酸熒光素標記) FCM
UMAB966Hu21 多配體蛋白聚糖1(SDC1)單克隆抗體 WB; IHC
UMAB966Hu23 多配體蛋白聚糖1(SDC1)單克隆抗體 WB
UMAB966Hu22 多配體蛋白聚糖1(SDC1)單克隆抗體 WB
UMAB966Hu24 多配體蛋白聚糖1(SDC1)單克隆抗體 WB
UMAB966Hu25 多配體蛋白聚糖1(SDC1)單克隆抗體 WB
URAB966Hu21 多配體蛋白聚糖1(SDC1)重組抗體 WB; IF; ICC; IHC; IP; FCM.
UMAB966Hu27 多配體蛋白聚糖1(SDC1)單克隆抗體 IHC
UMAB966Hu29 多配體蛋白聚糖1(SDC1)單克隆抗體 WB; IHC; ICC/IF
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USCB966Hu 多配體蛋白聚糖1(SDC1)檢測試劑盒(化學發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
ULMB966Hu 多配體蛋白聚糖1(SDC1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UKSB966Hu01 多配體蛋白聚糖1(SDC1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
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Mediators Inflamm Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding [PubMed: 26199464]
Transplantation Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury. [PubMed: 26371596]
Clinical and Experimental Nephrology Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury [pubmed:27405618]
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