細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體
Monoclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2)
MAPK1; P38; P40; ERK2; ERT1; MAPK2; P42MAPK; P42-MAPK; PRKM1; PRKM2; P41; P41mapk; P41-mapk; Mitogen-Activated Protein Kinase 1; Mitogen-activated protein kinase 2
- 編號UMAA930Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C1
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPA930Hu02-細胞外信號調(diào)節(jié)激酶2(ERK2)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Chicken (Gallus，雞)
- 應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 376 ¥ 878 ¥ 1254 ¥ 3135 ¥ 12540
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Porcine Lung Tissue; Primary Ab: 10μg/ml Mouse Anti-Human ERK2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;<br/>Sample: Human Thyroid cancer Tissue; <br/>Primary Ab: 20μg/ml Mouse Anti-Human ERK2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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DAB staining on IHC-P;<br/>Sample: Human Breast cancer Tissue; <br/>Primary Ab: 20μg/ml Mouse Anti-Human ERK2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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DAB staining on IHC-P;<br/>Sample: Human Colon Tissue; <br/>Primary Ab: 20μg/ml Mouse Anti-Human ERK2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Human Hela cell lysate Primary Ab: 2μg/ml Mouse Anti-Human ERK2 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate; Lane3: Mouse Cerebrum lysate; Lane4: Canine Cerebrum lysate; Lane5: Gallus Cerebrum lysate; Lane6: Bovine Cerebrum lysate; Lane7: Cavia Cerebrum lysate; Lane8: Caprine Cerebrum lysate
Primary Ab: 0.4μg/ml Mouse Anti-Human ERK2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對ERK2的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別ERK2。
用法
Western blotting: 0.01-2μg/ml;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
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參考文獻
| 雜志 | 參考文獻 |
| Cellular and Molecular Neurobiology | Hypericum Perforatum?Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease [Pubmed:26119304] |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome [pubmed:27538909] |
| International Journal of Reproductive Medicine | Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis [33575317] |
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