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抗微管蛋白β(TUBb)單克隆抗體

Anti-Tubulin Beta (TUBb) Monoclonal Antibody

TUB-B; TUBB5; TUBB1; M40

  • 編號UCAB870Mi22
  • 物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
  • 純化方式蛋白A + 蛋白G親和純化
  • 標記物無標記物
  • 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
  • 性狀液體
  • 濃度0.65mg/mL
  • 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Hamster (Chinese hamster，倉鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Chicken (Gallus，雞)
  • 應用WB; IHC; ICC/IF
  • 下載 英文說明書   中文說明書
  • 規(guī)格 20μl100μl 200μl 1ml 10ml
  • 價格 ¥ 358 ¥ 836 ¥ 1194 ¥ 2985 ¥ 11940
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  • 抗微管蛋白β(TUBb)單克隆抗體 產(chǎn)品包裝(模擬)
  • 抗微管蛋白β(TUBb)單克隆抗體 產(chǎn)品包裝(模擬)
  • 抗微管蛋白β(TUBb)單克隆抗體 DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • 抗微管蛋白β(TUBb)單克隆抗體 DAB staining on IHC-P;
    Sample: Human Colon Tissue;
    Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗微管蛋白β(TUBb)單克隆抗體 DAB staining on IHC-P;
    Sample: Human Cerebrum Tissue;
    Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗微管蛋白β(TUBb)單克隆抗體 DAB staining on IHC-P;
    Sample: Human Cardiac Muscle Tissue;
    Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗微管蛋白β(TUBb)單克隆抗體 DAB staining on IHC-P;
    Sample: Human Kidney Tissue;
    Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗微管蛋白β(TUBb)單克隆抗體 DAB staining on IHC-P;
    Sample: Human Lymphoma Tissue;
    Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗微管蛋白β(TUBb)單克隆抗體 DAB staining on IHC-P;
    Sample: Human Lung Tissue;
    Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗微管蛋白β(TUBb)單克隆抗體 FITC staining on IF;
    Sample: Human MCF7 cell;
    Primary Ab: 40μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu18)
  • 抗微管蛋白β(TUBb)單克隆抗體 FITC staining on IF;
    Sample: Human HepG2 cell;
    Primary Ab: 20μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu18)
  • 抗微管蛋白β(TUBb)單克隆抗體 AF488 staining on IF;
    Sample: HepG2 cell
    TRITC Phalloidin at 100 nM (shown in pseudo color red)
    Primary Ab: 20μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • CAB870Mi22.png Figure. Western Blot Sample: Recombinant TUBb
  • 抗微管蛋白β(TUBb)單克隆抗體 Western Blot analysis of TUBb in various cell lines
    Lane1: HL60 whole cell lysate
    Lane2: THP-1 whole cell lysate
    Lane3: MDA-MB-435S whole cell lysate
    Lane4: T-47D whole cell lysate
    Lysates/proteins at 20μg per lane.
    Primary Ab: anti-TUBb antibody (CAB870Mi22) at 0.3ng/ml
    Secondary Ab: HRP-conjugated Rabbit anti-mouse antibody (SAA544Mu09) at 1/10000 dilution
    Developed using the ECL technique.
    Calculated MW: 50KD
    Observed MW: 50KD
    Exposure time: 1 min
  • 抗微管蛋白β(TUBb)單克隆抗體 Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Canine Kidney lysate; Lane4: Gallus Cerebrum lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Cerebrum lysate; Lane7: Bovine Kidney lysate; Lane8: Rabbit Kidney lysate; Lane9: Cavia Kidney lysate; Lane10: Caprine Kidney lysate
    Primary Ab: 1μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗微管蛋白β(TUBb)單克隆抗體 Western Blot; Sample: Lane1: DU145 cell lysate; Lane2: HL60 cell lysate; Lane3: THP1 cell lysate; Lane4: OS-RC-2 cell lysate; Lane5: HEC-1B cell lysate; Lane6: MDA-MB-435S cell lysate; Lane7: T-47D cell lysate
    Primary Ab: 0.5μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗微管蛋白β(TUBb)單克隆抗體 Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Heart lysate; Lane3: Mouse Cerebellum lysate; Lane4: Porcine Cerebrum lysate; Lane5: Bovine Kidney lysate; Lane6: Rabbit Pancreas lysate
    Primary Ab: 0.0003μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗微管蛋白β(TUBb)單克隆抗體 Western Blot; Samples: Lane1: SH-SY5Y cell lysate; Lane2: Hela cell lysate; Lane3: HepG2 cell lysate; Lane4: PC3 cell lysate;
    Primary Ab: 0.1μg/ml Mouse Anti-Multi-species TUBb Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

該抗體是針對TUBb的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別TUBb。

用法

Western blotting: 0.0003-0.1ug/ml
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。

贈品

相關產(chǎn)品

編號 適用物種：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) 應用(僅供研究使用，不用于臨床診斷！)
UCAB870Mi01 抗微管蛋白β(TUBb)多克隆抗體 WB
UCAB870Mi22 抗微管蛋白β(TUBb)單克隆抗體 WB; IHC; ICC/IF
USEB870Mi 微管蛋白β(TUBb)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB870Mi 微管蛋白β(TUBb)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
archives of pharmacal research Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues [pubmed:27747473]
archiv der pharmazie (Weinheim). New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition. [pubmed:28150327]
ACS?Applied?Materials?&?Interfaces Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixture [pubmed:28112513]
Future?Medicinal Chemistry Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agents [Pubmed:29779400]
Future?Medicinal Chemistry Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerization [Pubmed:29966433]
Journal of Biological Chemistry Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167]
Journal?of?Enzyme?Inhibition?and?Medicinal?Chemistry Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2Inhibitors [Pubmed: 30191744]
European Journal of Medicinal Chemistry Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking study [Pubmed: 31536891]
ACS Sensors Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platform [Pubmed: 32207606]
Bioorganic Chemistry Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their?… [Pubmed: 32325332]
BRITISH JOURNAL OF NUTRITION Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats [Pubmed: 33028437]
European Journal of Medicinal Chemistry Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon?… [Pubmed: 33158579]
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Cancer Cell International Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins []
Cancer Cell Int Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212]
Sci Rep Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026]
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