運動因子相關(guān)蛋白1(MRP1)活性蛋白
Active Motility Related Protein (MRP1)
CD9; TSPAN29; 5H9; BA2; BTCC-1; DRAP-27; GIG2; MIC3; P24; Tetraspanin 29; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3
- 編號UAPB097Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點5.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 636 ¥ 1590 ¥ 3180 ¥ 9540 ¥ 23850
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Motility Related Protein (MRP1) is a member of the superfamily of ATP-binding cassette (ABC) transporters. It can enable ATPase-coupled inorganic anion transmembrane transporter activity. Involved in anion transmembrane transport ; renal tubular secretion ; and response to toxic substance. It is an integral component of plasma membrane. Besides, ITGb1 has been identified as an interactor of MRP1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MRP1 and recombinant human ITGb1. Briefly, biotin-linked MRP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to ITGb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of MRP1 and ITGb1 was shown in Figure 1, the EC50?for this effect is 0.049 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
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參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Nanomedicine | Upstream Hedgehog signaling components are exported in exosomes of cervical cancer cell lines [Pubmed: 30265222] |
| Biology-Basel | Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy [33374530] |
