基質金屬蛋白酶9(MMP9)活性蛋白
Active Matrix Metalloproteinase 9 (MMP9)
GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase
- 編號UAPA553Po01
- 物種Sus scrofa; Porcine (Pig，豬) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點4.8
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 886 ¥ 2214 ¥ 4428 ¥ 13284 ¥ 33210
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
Figure. Hydrolysis of gelatin by MMP9.<br/>Mechanism: MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant pig MMP9 (1000ng, 500ng, 100ng, 10ng, 1ng, 0.1ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate- polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP9 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control. <br/>Result: Gelatin hydrolysis by recombinant pig MMP9 (10-70kd) was shown in figure 1.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Sus scrofa; Porcine (Pig，豬) | 應用(僅供研究使用，不用于臨床診斷！) |
| UAPA553Po01 | 基質金屬蛋白酶9(MMP9)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA553Po61 | 基質金屬蛋白酶9(MMP9)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA553Po01 | 基質金屬蛋白酶9(MMP9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA553Po01 | 基質金屬蛋白酶9(MMP9)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAA553Po21 | 基質金屬蛋白酶9(MMP9)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA553Po | 基質金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA553Po | 基質金屬蛋白酶9(MMP9)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
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| МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ [] | |
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| Lab Invest | Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis [Pubmed: 32709888] |
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| JAMA Surgery | Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment [Pubmed: 28593326] |
| OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY | Intracranial Aneurysm Biomarker Candidates Identified by a Proteome-Wide Study [Pubmed: 32525733] |
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| International Journal of Pharmacology | Anti-arthritic Effects of Platelets Rich Plasma and Hyaluronic Acid on Adjuvant-induced Arthritis in Rats [] |
| Asian Journal of Pharmacy and Pharmacology | Evaluation of altered ground matrix and matrix metalloproeinase (MMP'S) in wound healing with Aloe vera extract [] |
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| Клиническая медицина | ДИНАМИКА МАРКЕРА ФИБРОЗИРОВАНИЯ ММР-9 У БОЛЬНЫХ ЛИМФОМОЙ ХОДЖКИНА [] |
| ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | Krill oil and low-dose aspirin combination mitigates experimentally induced silicosis in rats: role of NF-|êB/TGF-|?1/MMP-9 pathway [33398741] |
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| CLINICAL SCIENCE | Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes [33491733] |
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| DOCSBAY | Intermittent Pacing Therapy Favorably Modulates Infarct Remodeling [28386775] |
| Drug Chem Toxicol | Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline?… [34167392] |
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| CNS Neurosci Ther | Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke [34591349] |
| Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer [34650982] | |
| Disease Markers | Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study [34707724] |
| Medicina (Kaunas) | Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980] |
| Biomolecules | Increase in Serum MMP-9 and TIMP-1 Concentrations during Alcohol Intoxication in Adolescents—A Preliminary Study [Pubmed:35625637] |
| Journal of Pharmaceutical Research International | Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid?… [] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] | |
| Turkish Journal of Biochemistry-Turk Biyokimya Dergisi | Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes [] |
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