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巨噬細(xì)胞炎性蛋白1α(MIP1a)活性蛋白

Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)

CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein

  • 巨噬細(xì)胞炎性蛋白1α(MIP1a)活性蛋白 產(chǎn)品包裝(模擬)
  • 巨噬細(xì)胞炎性蛋白1α(MIP1a)活性蛋白 產(chǎn)品包裝(模擬)
  • APA092Mu61.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis?assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers and various concentrations of MIP-1a was added in lower chamber with a polycarbonate filter (8 um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted using Image J. The migrated THP1 cells in low chamber at low magnification (×100) was shown in Figure 1. The number of migrated THP-1 cells was shown in Figure 2. The optimum chemotaxis of MIP-1a occurs at 1 ug/ml.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA092Mu01 巨噬細(xì)胞炎性蛋白1α(MIP1a)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPA092Mu61 巨噬細(xì)胞炎性蛋白1α(MIP1a)活性蛋白 Cell?culture;?Activity?Assays.
URPA092Mu02 巨噬細(xì)胞炎性蛋白1α(MIP1a)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UEPA092Mu61 巨噬細(xì)胞炎性蛋白1α(MIP1a)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAA092Mu01 巨噬細(xì)胞炎性蛋白1α(MIP1a)多克隆抗體 WB
UPAA092Mu02 巨噬細(xì)胞炎性蛋白1α(MIP1a)多克隆抗體 WB; IHC; ICC; IP.
ULAA092Mu71 巨噬細(xì)胞炎性蛋白1α(MIP1a)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
UMEA092Mu 巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
USEA092Mu 巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA092Mu 巨噬細(xì)胞炎性蛋白1α(MIP1a)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UCGA092Mu01 巨噬細(xì)胞炎性蛋白1α(MIP1a)cDNA PCR、蛋白表達(dá)等

參考文獻(xiàn)

雜志 參考文獻(xiàn)
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National Natural Scientific Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure [pubmed:29121396]
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Journal of Immunology?Research Cathelicidin LL-37 affects surface and intracellular Toll-like receptor expression in tissue mast cells [Pubmed:29670923]
Pharmacological Reports Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without?… [Pubmed:29339257]
Journal?of?Food?Science CQPC06 Activity Prevents Dextran Sulfate Sodium‐Induced Colitis by Regulating the IL‐8 Pathway [Doi: 10.1111/1750-3841.14346]
Biochemical and Biophysical Research Communications The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusion [Pubmed: 30558792]
LIFE SCIENCES Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stress [Pubmed: 33227274]
ACTA PHARMACOLOGICA SINICA α-Galactosylceramide and its analog OCH differentially affect the pathogenesis of ISO-induced cardiac injury in mice [Pubmed: 32973325]
Life Sci PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escape [Pubmed: 32971102]
Nature Communications A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy [Pubmed: 32332752]
Neoplasia PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1 [34325342]
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