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吲哚胺2,3-雙加氧酶(IDO)活性蛋白

Active Indoleamine-2,3-Dioxygenase (IDO)

CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase

  • 吲哚胺2,3-雙加氧酶(IDO)活性蛋白 產(chǎn)品包裝(模擬)
  • 吲哚胺2,3-雙加氧酶(IDO)活性蛋白 產(chǎn)品包裝(模擬)
  • 吲哚胺2,3-雙加氧酶(IDO)活性蛋白 Figure . Gene Sequencing (extract)
  • APB547Hu01.jpg Figure. SDS-PAGE; Sample: Active recombinant IDO, Human.
  • 吲哚胺2,3-雙加氧酶(IDO)活性蛋白 Figure. Western Blot; Sample: Recombinant IDO, Human.
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

IDO (Indoleamine 2,3-dioxygenase 1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. Thus, bioactivity of recombinant human IDO was measured through its ability to oxidize L-tryptophan to N-formyl-kynurenine, using Methylene Blue as indicator. The reaction was performed in 50 mM MES, pH 6.5 (Assay Buffer), initiated by addition 50 μL of various concentrations of IDO (diluted by Assay Buffer) to 50 μL substrate mixture of 800 μM L-tryptophan, 9000 units/mL catalase （RPC418Hu05）, and 40 μM Methyene Blue in assay buffer with equal volume of 80 mM ascorbic acid in 0.405 M Tris, pH 8.0. The final well serves as a negative control with no IDO, replaced with 50μL assay buffer. The absorbance was read in 321 nm in kinetic mode for 5 minutes. The result indicated that recombinant human IDO can oxidize L-tryptophan, the specific activity is 10581 pmol/min/μg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPB547Hu01 吲哚胺2,3-雙加氧酶(IDO)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPB547Hu01 吲哚胺2,3-雙加氧酶(IDO)活性蛋白 Cell?culture;?Activity?Assays.
UPAB547Hu01 吲哚胺2,3-雙加氧酶(IDO)多克隆抗體 WB; IHC; ICC/IF
ULAB547Hu71 吲哚胺2,3-雙加氧酶(IDO)多克隆抗體(生物素標記) WB
ULAB547Hu81 吲哚胺2,3-雙加氧酶(IDO)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
UMAB547Hu22 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 WB; ICC/IF; FCM
UMAB547Hu24 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 ICC/IF
UMAB547Hu21 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 WB; IHC
UMAB547Hu23 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 WB
UFAB547Hu08 抗吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 FCM
UFAB547Hu06 抗吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 FCM
UFAB547Hu04 抗吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 FCM
UFAB547Hu02 抗吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 FCM
ULAB547Hu72 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體(生物素標記) WB; IHC; ICC.
USEB547Hu 吲哚胺2,3-雙加氧酶(IDO)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB547Hu 吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UPSB547Hu01 吲哚胺2,3-雙加氧酶(IDO)抗體對 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
UKSB547Hu01 吲哚胺2,3-雙加氧酶(IDO)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Blood CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects [Pubmed: 21803856]
Clinical Transplantation Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT [Wiley: source]
Indonesian Journal of Cancer Chemoprevention Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell [Iregway: Source]
PLoS ONE CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms [PubMed: PMC3683035]
Hepatology Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma [Pubmed: 23960017]
Free Radical Biology and Medicine Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans [ScienceDirect: S0891584913006345]
Frontiers in Oncology Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation [Frontiersin:Source]
Tissue Engineering Part A Immunogenicity and escape mechanisms of allogeneic tendon-derived stem cells [Pubmed:24813640]
Immunobiology. The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered [Pubmed:24947894]
Apoptosis Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes [Pubmed:25577277]
Front Oncol Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation [PubMed: 26029659]
Acta Universitatis Tamperensis Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties [Handle: 10024]
PLoS One Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase [pubmed:27695127]
Journal of Biomedical Nanotechnology Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility [Pubmed:29883557]
Biochemistry Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells []
American Journal of Pathology Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD Mice [Pubmed: 30980800]
international immunopharmacology Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression [Pubmed: 31176083]
Stem Cell Reports Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4 []
International Journal of?Tryptophan?Research Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes??- [33343199]
Stem Cell Reports Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4 []
Transl Psychiatry Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive?… [34802039]
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