凝血因子Ⅱ(F2)活性蛋白
Active Coagulation Factor II (F2)
FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2
- 編號UAPA820Ra02
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點4.9
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Gene sequencing
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant F2, Human.
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
Coagulation Factor II (F2) also commonly called pro-thrombin is a coagulation protein in the blood stream that has many effects in the coagulation cascade. It is a serine protease that converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Besides, Protein C Inhibitor (PCI) has been identified as an interactor of F2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat F2 and recombinant rat PCI. Briefly, F2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PCI-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-F2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of F2 and PCI was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA820Ra03 | 凝血因子Ⅱ(F2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA820Ra02 | 凝血因子Ⅱ(F2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA820Ra01 | 凝血因子Ⅱ(F2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA820Ra02 | 凝血因子Ⅱ(F2)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA820Ra02 | 凝血因子Ⅱ(F2)多克隆抗體 | WB; IHC; ICC/IF |
| UPAA820Ra03 | 凝血因子Ⅱ(F2)多克隆抗體 | WB; IHC |
| UPAA820Ra01 | 凝血因子Ⅱ(F2)多克隆抗體 | WB; IHC |
| UFAA820Ra01 | 抗凝血因子Ⅱ(F2)多克隆抗體 | FCM |
| ULAA820Ra71 | 凝血因子Ⅱ(F2)多克隆抗體(生物素標記) | WB |
| ULAA820Ra81 | 凝血因子Ⅱ(F2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UMAA820Ra26 | 凝血因子Ⅱ(F2)單克隆抗體 | ICC/IF |
| UMAA820Ra29 | 凝血因子Ⅱ(F2)單克隆抗體 | WB; IHC |
| UMAA820Ra21 | 凝血因子Ⅱ(F2)單克隆抗體 | WB; IHC |
| UMAA820Ra23 | 凝血因子Ⅱ(F2)單克隆抗體 | WB; IHC |
| UMAA820Ra24 | 凝血因子Ⅱ(F2)單克隆抗體 | WB; IHC |
| UMAA820Ra25 | 凝血因子Ⅱ(F2)單克隆抗體 | WB; IHC |
| UMAA820Ra27 | 凝血因子Ⅱ(F2)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA820Ra22 | 凝血因子Ⅱ(F2)單克隆抗體 | WB; IHC |
| USEA820Ra | 凝血因子Ⅱ(F2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA820Ra | 凝血因子Ⅱ(F2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA820Ra01 | 凝血因子Ⅱ(F2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Blood Cells Mol Dis | Upregulation of cytokine signalling in platelets increases risk of thrombophilia in severe COVID-19 patients [Pubmed:35180460] |
