17-β-羥基類固醇脫氫酶14(HSD17b14)活性蛋白
Active 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14)
DHRS10; RetSDR3, SDR47C1; Retinal Short-Chain Dehydrogenase/Reductase 3; Short Chain Dehydrogenase/Reductase Family 47C,Member 1
- 編號(hào)UAPF175Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)5.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià)
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) is an enzyme mainly expressed in the liver and other tissues. It belongs to the hydroxysteroid dehydrogenase family and participates in the metabolism of steroid hormones. HSD17b14 can catalyze the interconversion of specific steroid substrates, influencing the balance of steroid hormone levels in the body.Brsides,HSD17b14 can regulate cellular pH and metabolic processes by binding to Carbonic Anhydrase VIII (CA8), affecting activities related to steroid metabolism and ion balance to maintain cellular stability.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human HSD17b14 and recombinant human CA8 . Briefly, biotin-linked HSD17b14 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to CA8-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of HSD17b14 and CA8 was shown in Figure 1, the EC50?for this effect is 0.23ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPF175Hu01 | 17-β-羥基類固醇脫氫酶14(HSD17b14)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPF175Hu01 | 17-β-羥基類固醇脫氫酶14(HSD17b14)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAF175Hu01 | 17-β-羥基類固醇脫氫酶14(HSD17b14)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAF175Hu21 | 17-β-羥基類固醇脫氫酶14(HSD17b14)單克隆抗體 | WB; IHC; ICC; IP. |
| USEF175Hu | 17-β-羥基類固醇脫氫酶14(HSD17b14)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMF175Hu | 17-β-羥基類固醇脫氫酶14(HSD17b14)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Archives of Toxicology | Bisphenol A?induces DSB-ATM-p53 signaling leading to?cell cycle arrest, senescence, autophagy, stress response, and?estrogen release in?human fetal lung fibroblasts [/s00204-017-2150-3] |
| Archives?of?Toxicology | Bisphenol A induces DSB-ATM-p53 signaling leading to cell cycle arrest, senescence, autophagy, stress response, and estrogen release in human fetal lung?… [Pubmed:29275510] |
| Anticancer Research | Role of Some Enzymes in Early Diagnosis of Ovarian Cancer [Pubmed: 29848718] |
