免疫球蛋白E(IgE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Immunoglobulin E (IgE)
IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region
- 編號UKSA545Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍1.56-100ng/mL
- 可適靈敏度0.69ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 3402 ¥ 5670 ¥ 10206 ¥ 19845 ¥ 34020
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對免疫球蛋白E(IgE)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UNPA545Hu01 | 免疫球蛋白E(IgE)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA545Hu61 | 免疫球蛋白E(IgE)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA545Hu01 | 免疫球蛋白E(IgE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA545Hu01 | 免疫球蛋白E(IgE)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA545Hu01 | 免疫球蛋白E(IgE)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA545Hu71 | 免疫球蛋白E(IgE)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| URAA545Hu21 | 免疫球蛋白E(IgE)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA545Hu22 | 免疫球蛋白E(IgE)單克隆抗體 | WB |
| UMAA545Hu21 | 免疫球蛋白E(IgE)單克隆抗體 | WB |
| UMAA545Hu23 | 免疫球蛋白E(IgE)單克隆抗體 | WB |
| USEA545Hu | 免疫球蛋白E(IgE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA545Hu | 免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA545Hu01 | 免疫球蛋白E(IgE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| AAPS PharmSciTech | Topical Lyogel Containing Corticosteroid Decreases IgE Expression and Enhances the Therapeutic Efficacy Against Atopic Eczema [PubMed: 25511806] |
| Ann Allergy Asthma Immunol | Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation [PubMed: 26123423] |
| Molecular Medicine Reports | The?protective?role?of?vitamin?D3?in a?murine?model?of?asthma?via?the?suppression?of TGF-β/Smad?signaling?and?activation?of the?Nrf2/HO-1?pathway. [pubmed:27484042] |
| Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847] |
| International Immunopharmacology | Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung [pubmed:27992791] |
| Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397] |
| Molecular Medicine Reports | Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. [pubmed:28586019] |
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| INTERNATIONAL JOURNAL OF MOLECULAR?SCIENCES? | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
| Royal?Society?Open?Science | Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats [] |
| Health and Medicine | Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis [] |
| American Journal of Translational Research | Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193] |
| MOLECULAR IMMUNOLOGY | Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806] |
| bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
| international journal of antimicrobial agents | Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in Vivo [Pubmed: 32335278] |
| Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota [] | |
| ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction [] |
| Biomarkers of Allergic Asthma and their Association with Serum Parameters [] | |
| Biomed Pharmacother | Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice [33706133] |
| Environ Pollut | Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure [34090073] |
| Annals of Tropical Medicine & Public Health | Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity [] |
| Mediators Inflamm | Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal Polyps [Pubmed:35075349] |
| Expert Opinion on Therapeutic Targets | Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma [Pubmed:35549595] |
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