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細(xì)胞周期素D1(CCND1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Cyclin D1 (CCND1)

CCN-D1; BCL1; PRAD1; U21B31; G1/S-Specific Cyclin-D1; Parathyroid Adenomatosis 1; B-Cell CLL/Lymphoma 1; G1/S-Specific Cyclin D1; PRAD1 oncogene

  • 細(xì)胞周期素D1(CCND1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 細(xì)胞周期素D1(CCND1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對細(xì)胞周期素D1(CCND1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA585Hu01 細(xì)胞周期素D1(CCND1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPA585Hu02 細(xì)胞周期素D1(CCND1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAA585Hu01 細(xì)胞周期素D1(CCND1)多克隆抗體 WB; ICC/IF
UMAA585Hu22 細(xì)胞周期素D1(CCND1)單克隆抗體 WB; IHC; ICC; IP.
USEA585Hu 細(xì)胞周期素D1(CCND1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA585Hu 細(xì)胞周期素D1(CCND1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UKSA585Hu01 細(xì)胞周期素D1(CCND1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Biochemical Pharmacology Antiproliferative and proapoptotic activity of CLM3, a novel multiple tyrosine kinase inhibitor, alone and in combination with SN-38 on endothelial and cancer cells [ScienceDirect: S0006295211002097]
Leukemia & Lymphoma The nanoparticulate Quillaja saponin KGI exerts anti-proliferative effects by down-regulation of cell cycle molecules in U937 and HL-62 human leukemia cells [Pubmed: 24138332]
The Journal of Clinical Endocrinology and Metabolism Antiproliferative and proapoptotic activity of sunitinib on endothelial and anaplastic thyroid cancer cells via inhibition of Akt and ERK1/2 phosphorylation and by down-regulation of cyclin-D1. [Pubmed: 23969186]
Nutrition and Cancer Common Food Additive Carrageenan Stimulates Wnt/ β-Catenin Signaling in Colonic Epithelium by Inhibition of Nucleoredoxin Reduction [Pubmed:24328990]
The International Journal of Biochemistry &?Cell?Biology MicroRNA-149 is epigenetically silenced tumor-suppressive microRNA, involved in cell proliferation and downregulation of AKT1 and cyclin D1 in human … [pubmed:27783537]
Scientific Reports Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA. [pubmed:28533512]
Investigational new drugs Development of a dietary formulation of the SHetA2 chemoprevention drug for mice. [pubmed:29273857]
Biochemical Pharmacology Metronomic vinorelbine is directly active on Non Small Cell Lung Cancer cells and sensitizes the EGFRL858R/T790M cells to reversible EGFR tyrosine kinase?… [Pubmed:29660315]
Apoptosis Licarin A induces cell death by activation of autophagy and apoptosis in non-small cell lung cancer cells [Pubmed:29468481]
Oncology?Reports Vandetanib has antineoplastic activity in anaplastic thyroid cancer, in vitro and in vivo [Pubmed:29517106]
Oncology Reports Lenvatinib exhibits antineoplastic activity in anaplastic thyroid cancer in vitro and in vivo [Pubmed:29517103]
Scientific Reports Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA [Pubmed: 28533512]
Middle East Journal of Rehabilitation and Health Antiapoptotic Effects of Continuous Training and Selenium Consumption on the Liver Tissue of Cadmium-Exposed Rats [Pubmed: 10.5812/mejrh.91278]
European journal of medicinal chemistry Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity [Pubmed: 31216504]
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